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Broad Immune Adjuvant

  • Broad Immune Adjuvant
    • Broad Immune Adjuvant
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    • Broad Immune Adjuvant
      Cat No.:KYSJ-A003/4

      Application: Animal Immunization Preparation Antibody

      Term of validity: This product is stable for 2 years stored at 2°C~8°C. Shading light.

      All of our products are only available to industrial customers or research institutes for experimental use, not for other applications!
       
      Broad Immune Adjuvant
       
      Broad Immune Adjuvant is made by screening the ingredients that produce more effective stimulation of the immune system under the guidance of the principle of Freund's adjuvant. Using this adjuvant, the specific anti-serum in the animal anti-serum obtained was more than twice as high as the specific IgG obtained using Freund's adjuvant (reflected in antiserum titer). Whether the antibody obtained by immunization can be used for WB, IHC, IF, IP, ELISA, etc., is affected by the design of the antigen, and is also affected to a large extent by the quality of the anti-serum obtained by immunization. In general, when the antiserum titer is low, the quality of the purified IgG is also greatly affected. With the wide adjuvant, not only the titer of antiserum was increased by more than 2 times, but also the immune cycle was shortened from 70 days of Freund's adjuvant to 40 days. The following are the specific methods of use:
       
      Immunization Method:
        Adjuvant Name Immunity Immunization Time Immune Position and Number of Immunization Points
      First Immunization Immune Adjuvant A (Emulsified with an equal volume of antigen) Each rabbit/mouse is immunized with 1 mg/0.2 mg of antigen,and the immune volume is around 1ml/0.2ml The 1st Day Subcutaneous injection of 5 points or more in the back (intraperitoneal injection of mice)
      Second Immunization Immune Adjuvant B (Emulsified with an equal volume of antigen) Each rabbit/mouse is immunized with 1mg/0.2mg antigen, and the immune volume is around 1ml/0.2ml. 30th Day Subcutaneous injection of 5 points or more in the back (intraperitoneal injection of mice)
      Execute & Blood Sample Collection.     40th Day  






         
       



       







      Note:
      Shake well before using the adjuvant.
       
      Storage Method:
      Immune Adjuvant A - 2-8 degrees Celsius, valid for 2 years.
      Immune Adjuvant B - 2-8 degrees Celsius, valid for 2 years.

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